,Perrimon_Hidde Ploegh Labs, - Donated Vectors,,,,,,

DGRC Bar Code #,Plasmid Name,Purpose,Gene or insert Name,N-terminal Tag,C-terminal Tag,Backbone Name,Bacterial Resistance,Growth Strain
1550,pMT-HGP-v3-Nb127D01-hIgG,Nanobody-hIgG expression in fly cell,Nb127D01-human IgG-TEV-Avi-His,BiP signal peptide,hIgG1-hinge-N297A-TEV site/Avi-Tag/His-Tag,pMT-HGP-v3,Ampicillin,DH5alpha
1551,pMT-HGP-v3-NbVHH05-hIgG,Nanobody-hIgG expression in fly cell,NbVHH05-human IgG-TEV-Avi-His,BiP signal peptide,hIgG1-hinge-N297A-TEV site/Avi-Tag/His-Tag,pMT-HGP-v3,Ampicillin,DH5alpha
1552,pET-26b-Nb127D01-HA-His,Nanobody-HA-His expression in bacteria,Nb127D01-HA-His,PelB signal peptide,HA-tag/His-tag,pET-26b,Kanamycin,DH5alpha
1553,pET-26b-NbVHH05-HA-His,Nanobody-HA-His expression in bacteria,NbVHH05-HA-His,PelB signal peptide,HA-tag/His-tag,pET-26b,Kanamycin,DH5alpha
1554,pET-26b-Nb127D01-ALFA-His,Nanobody-ALFA-His expression in bacteria,Nb127D01-ALFA-His,PelB signal peptide,ALFA-tag/His-tag,pET-26b-Nb-GGA,Kanamycin,DH5alpha
1555,pET-26b-NbVHH05-ALFA-His,Nanobody-ALFA-His expression in bacteria,NbVHH05-ALFA-His,PelB signal peptide,ALFA-tag/His-tag,pET-26b-Nb-GGA,Kanamycin,DH5alpha
1556,pAW-NbVHH05-GFP,Express NbVHH05 fused GFP in fly cell,NbVHH05-GFP,,GFP,pAW,Ampicillin,DH5alpha
1557,pAW-Nb127D01-GFP,Express Nb127D01 fused GFP in fly cell,Nb127D01-GFP,,GFP,pAW,Ampicillin,DH5alpha
1558,pAW-NbVHH05-mCherry,Express NbVHH05 fused mCherry in fly cell,NbVHH05-mCherry,,mCherry,pAW,Ampicillin,DH5alpha
1559,pAW-Nb127D01-mCherry,Express Nb127D01 fused mCherry in fly cell,Nb127D01-mCherry,,mCherry,pAW,Ampicillin,DH5alpha
1560,pAW-BiP-NbVHH05-mCherry-KDEL,Express NbVHH05 fused mCherry in fly cell ER membrane,BiP-NbVHH05-mCherry-KDEL,BiP signal peptide,mCherry-KDEL,pAW,Ampicillin,DH5alpha
1561,pAW-BiP-Nb127D01-mCherry-KDEL,Express Nb127D01 fused mCherry in fly cell ER membrane,BiP-Nb127D01-mCherry-KDEL,BiP signal peptide,mCherry-KDEL,pAW,Ampicillin,DH5alpha
1562,pAW-CD8-NbVHH05-GFP,Express NbVHH05 fused GFP in fly cell cytomembrane,CD8-NbVHH05-GFP,CD8,GFP,pAW,Ampicillin,DH5alpha
1563,pAW-CD8-Nb127D01-GFP,Express Nb127D01 fused GFP in fly cell cytomembrane,CD8-Nb127D01-GFP,CD8,GFP,pAW,Ampicillin,DH5alpha
1564,pScarlessHD-C-3x127D01-DsRed,Vector containing a cassette for scarless insertion of 3x127D01 into C-terminal and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.,3x127D01,,,pScarlessHD,Ampicillin,DH5alpha
1565,pScarlessHD-N-3x127D01-DsRed,Vector containing a cassette for scarless insertion of 3x127D01 into N-terminal and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.,3x127D01,,,pScarlessHD,Ampicillin,DH5alpha
1566,pScarlessHD-C-3xVHH05-DsRed,Vector containing a cassette for scarless insertion of 3xVHH05 into C-terminal and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.,3xVHH05,,,pScarlessHD,Ampicillin,DH5alpha
1567,pScarlessHD-N-3xVHH05-DsRed,Vector containing a cassette for scarless insertion of 3xVHH05 into N-terminal and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.,3xVHH05,,,pScarlessHD,Ampicillin,DH5alpha
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